#SpectraMax L Config) Methods. It also allows for the elimination of standard curves. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. A. 2022 Harini Natarajan et al. 0 OD at. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. Features. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. Related Products: Low Binding Plates. Microplates . In addition, SoftMax Pro Software In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. Abstract and Figures. While they play an important role in generating data from a wide range of. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. The SpectraMax Plus 384 Microplate Reader is a good choice for running red wine phenolics assay described by Heredia et al. Determine DNA concentrations precisely and . Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. 984. *Simple analysis and optimization. the assay. 0 H x 60. Incubate the plate at 37 °C for approximately one hour. The contents of the SpectraDrop Microplate kit depend on kit type. VANTAstar™ Flexible Multi-mode Microplate Reader. flat meniscus), but is approx 15 mL. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). ODs were measured in 96 well plates with a plate reader (Molecular Devices, SpectraMax ABS Plus) with two technical replicates of each of. flexibility of monochromator-based systems. You can use 6, 12, 24, 48, 96, and 384-well plates that conform to ANSI/SBS standard microplate footprint and dimensions in the instrument. Instrument settings can be saved as a protocol file and used repeatedly for reading differentMolecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. Highly selective for dsDNA over ssDNA or RNA. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Contents of wells were pipetted up and down several times to mix. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate. Infinite 200 Pro Microplate Reader. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. Important things to consider when purchasing a multimode microplate reader. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. 3. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Our microplate. SpectraMax Microplate Readers. 4 Pages. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. This reader also makes use of a unique temperature-independent methodology for path length correction. 96, 384, 24, 12, 6 well types. Simple, economical . Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. #PLUS 384) Methods. Read one sample or up to 384 in a. AquaMax 4000 Microplate Washer (Molecular Devices Cat. added to 96-well at 37 °C for 2 h, then the absorbance was measured at 450 nm by a. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. ± 0. When reading at wavelengths. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. Please refer to the Obsolete Microplate Reader Models for more information. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. Published Dec 16, 2020 | Updated Dec 16, 2020. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. The reader is ideal for measuring flash and glow assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer (BRET), and acridinium ester flash assays, in both 96- and 384-well plates. Paradigm: Delta mP. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. a white 96-well half-area microplate (Corning cat. Timothy Bolus, MPM, MBA, Compliance Program Manager. Learn more 0200-7008 6- to 1536-well plates. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Model:. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. (FP) configured microplate readers. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Microplate luminescent reader assay for ERK activity. Description. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Plate readers, also known as microplate readers or microplate photometers,. for ValitaTiter Assays. Published Dec 16, 2020 | Updated Dec 06, 2021. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. 125 pM. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. Microplate readers can be either single-function instruments or “multimode. Optical System: High energy xenon flash lamp. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. Products : General, FilterMax F5 reader. #N207) LAL Reagent Water (Lonza cat. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. Microplate luminescent reader assay for ERK activity. field-upgradeable option with one-of-a-kind brightfield cell segmentation, green and red. 747. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Retrieving. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. SpectraMax 250 User. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. 19 H mm that meet microplate industry standards. Align the two slots on the bottle holder with the two pegs on the rail. Wide range of applications The VersaMax reader covers. Outer wells are aligned for proper viewing with FLIPR. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. High. The SpectraDrop Microplate’s slide design eliminates the need for calibration and gives consistent well-to-well reads with CVs below 5%. ( a ) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. A. D. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 375 pM. We would like to show you a description here but the site won’t allow us. Catalog Number: (10822-512) Supplier: Molecular Devices Description: Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. Molecular Devices' microplate readers. 3. OEM Molecular Devices All Microplate Reader, Top r. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. For more sample throughput, both readers can be easily integrated into full roboticVisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath: Datapaq Express (Vial Rack Bottom Label 2D Reader). SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Key applications: ELISA, nucleic acid, endotoxin testing. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. Detection by Low noise PMT with simultaneous digital and analog modes. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. 1. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Description. SpectraMax® Microplate Reader Suite of Products Knowledge Base. Seller assumes all responsibility for this listing. . Filters: Excitation and emission slides for 4 filters each. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. 69: 85. ©2010 molecular Devices, inc. Cells were loaded with 2 µM Fluo-4 for one hour. The VersaMax Absorbance Microplate Reader measures the optical density (OD) of samples at selected wavelengths in a number of reading modes. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. Figure 2-23: Bottle holder To install the bottle holder: 1. g. M5. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 00. 100-μL samples ranging from 1 nM to 10 μM were added to triplicate wells of a solid white 96-well microplate. Microplate Readers. Microplate Formats: 6, 12, 24, 48, 96, 384. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. 54: 1. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. plate height between 12–16 mm and 96-well “strip” plates Barcode libraries Interleaved 2 of 5, Code 128, EAN 128, Code. Average StDev (mP) 1. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. ’s GloMax®-96, for instance, is a dedicated luminometer, whereas Bio-Rad Laboratories’ iMark and xMark strictly measure absorbance. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. A microplate reader as . Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. or ValitaTiter Plus (Molecular Devices cat. Biochemical characterization was conducted, using GEN III MicroPlate™ Biolog identification system and confirmed with molecular identification via 16S rDNA gene. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. Expt#3 . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. 3. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Consult Molecular Devices's entire Threshold Immunoassay System catalogue on MedicalExpo. Preconfigured protocols and custom assay workflows simplify microplate data acquisition and analysis. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. It includes both shaking and incubation functions, and is compatible with 96- well microplates. Built around a grating monochromator, the VersaMax™ Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. #ID3-STD) L-Tryptophan (Sigma cat. It measures the optical density (OD) of samples in both 96- and 384-well. Page 11: Reader ComponentsBioTek Synergy HTX Microplate Reader - Absorbance Only Specifications. 3% in white 96- and 384-well microplates Time-Resolved Fluorescence Performance (M4, M5, M5e only) Reading capabilities Top or bottom of a microplatethe iD5 reader’s filter system. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. Fluorescence Microplate Reader Comparison Chart. Omnia® Compatible Microplate Reader Documentation Version No. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Use standard plate unless you see your type listed. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. # 651101) the iD5 reader’s filter system. tools to expand the boundaries of their research. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Shaking: Linear and Orbital. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. . With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Note: SoftMax Pro Software must be installed on a computer connected to the instrument. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. microplate readers. This can increase throughput and efficiency, thus saving time and space in the lab. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 0200-6262. Paradigm: Delta mP. #1402-9700) 384-well low volume solid black microplate (Corning cat. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. 2 mm x 86 mm) Height:. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aPlus Microplate Reader, the R2 value was 0. #I2511)Introduction. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. Reader. The iMark. Reconstitution of. 0 500 1000 1500 2000 2500 3000 3500 time (sec. The spectrophotometer has a wide wavelength range 190. Published Dec 16, 2020 | Updated Dec 16, 2020. The kinetic top-read mode of a SpectraMax M3 microplate reader (Molecular Devices, CA, USA). Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. 1700 Online: Sales Offices. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity assays. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. CloneMatrix. 500 USD. 5°C at 37°C. Instrument settings are listed in Table 1. MycoAlert Reagent was added to each well and incubated at room temperature for 5. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit (signal 3X SD of baseline) 8. 5. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. 69: 85. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. best-in-class data . Temperature Control: ambient from +4 to 45C. 04 sec. Top or bottom detection is available with a simple click of a button. Molecular. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. Bioz Stars score: 86/100, based on 1 PubMed citations. Vmax Kinetic Microplate Reader with SOFTmax® Pro from Molecular Devices, now part of MDS Analytical Technologies. • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. Formats available are strip washers, full plate washers and combination washer-dispensers. It features a modular design that allows users to add or change detection cartridges according to their needs. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 0 mm Holex HEXAGON ballhead bolt driver to loosen the screw located inside the Transport Lock opening until you feel the spring release. Create custom fluorescence assays without having to purchase expensive filter sets. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Industry standard, 96-well plates are. Wavelength Range 200 to 999 nm. Three kits covering a wide range of dsDNA concentrations. All stand-alone instrument functions can be controlled using the software. explore more. < 0. Contents of wells were pipetted up and down several times to mix. 98 VMax R2 = 0. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. Figure 2. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. Type: Microplate Reader. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. buffers for (2) 96-well microplates R8088 CatchPoint cAMP 96-well Fluorescent Assay Kit (Bulk) ELISA-based assay for the detection and quantification of cAMP * Contains microplates, reagents, and buffers for (10) 96-well microplates R8089 SpectraMax® i3/i3x Multi-Mode Microplate Reader SpectraMax® Paradigm® FlexStation® 3 Multi-Mode. The assay kit is optimized for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. and luminescence microplate reader validation DATA SHEET. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. Microplate readers compatible with 96-well plates Single- to multi-mode readers combine an absorbance plate reader , a luminescence plate reader , and a fluorescence plate. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Fluorescence Microplate Reader Comparison Chart. San Jose, California, June 5, 2018. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Other features and benefits include: Eliminate changing out filters. 1 sec. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. In addition, SoftMax Pro Software Molecular Devices SpectraMax ® Paradigm ® Multi-Mode Microplate Detection Platform is a user upgradeable microplate reader that allows for real-time system configuration by the user in less than two minutes. 384 well polystyrene microplates have plate dimensions of 127. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. ValitaTiter Plus 96-well (1 plate) VAL004. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. Pick the best speed, sensitivity, and price for your needs. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Up to 70% savings in overall assay time can be gained when using the Cell Cytotoxicity assay (Figure 1). U-bottom: 30-40 µl. For more sample throughput, both readers can be easily integrated into full roboticThe SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The CellTiter-Glo assay from Promega uses luciferase enzyme. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. Integrated stacker option. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. for ValitaTiter Plus standards read on Molecular Devices readers. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. i3x. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Both readers were heated to 37ºC prior to setting up . For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. With optimized reagents, option to operate the. 1 OD at 230 nm. Wavelength Range. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). #VAL003 or VAL013) or ValitaTiter Plus (Molecular Devices cat. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. 6:30 . Cross-talk < 0. When the user applies a set temperature of 37°C, the top is heated to 37. 1 mL/well of media. Use the provided 3. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. 54: 1. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The procedure enables direct translation of any published equation. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. Description. $489. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. absorbance, fluorescence, and luminescence with the added functionality of. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. g. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all. M3-M5e_manual. Vrla used the device to quantify the concentration of a small molecule produced by. Temperature control. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. 7°C and the bottom to 36. Wavelength Selection. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. It features a modular design that allows users to add or change detection cartridges according to their needs. New SpectraMax iD5 Multi-mode Microplate Reader. Four wash/rinse bottles are included and configurable for both 96- and 384. Page 154: Ordering Parts And Accessories. Transfer of an assay to 96-well microplate technology increases throughput significantly. Page: 1/4. 8 Pages. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Compact design Minimum volume and. SpectraMax® M3/M4/M5/M5e, GeminiTM EM/XPS, FlexStation® 3. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability.